Improving viticulture ecosystems sustainability over the application of organic fertilizers with various bio-safe stimulants is the main objective of this work to obtain high yield and quality with high income. Therefore, this trial was conducted in 2020 and 2021 after a preliminary season 2019 to highlight the role of the organic fertilizer Vermicompost (VC) associated with two types of biostimulants namely ArbuscularMycorrhizal Fungi (AMF) at 150 & 300 g/vine, and three Plant Growth Promoting Rhizobacterial (PGPR) strains at 50% &100%, or the natural fertilizer Potasssium Humate Gel (PHG) at 50 &100 %. All combinations were applied in the presence of 50% of the vineyard basic mineral fertilization on seven years-old Flame Seedless cv. grapevines planted in a sandy loamy soil in a private degraded vineyard located at 58 km Cairo- Alexandria desert road, Egypt. Moreover, all the treated vines were sprayed twice with VC extract at two phonological stages, when cluster length about 10-15 cm and when berry diameter reached about 7-8 mm for boosting vegetative growth, nutritional status, yield and fruit quality. The obtained results indicated that VC associated with various stimulators half doses 50% or fully ones 100% had an affirmative role in improving all the parameters over the control vines. The clear superiority of these responses were scored by mixing VC soil applications plus with PGPR 100% which was more favorable in stimulating growth parameters, physical and chemical characteristics of berries, yield and nutritional status. However, the co-application of VC plus 100% PHG had distinctive effect on total leaf chlorophyll content (SPAD). Thus, there is a wide range of organic and biostimulants products which considered as a valuable choice to be investigated for decreasing the harmful impact of synthetic fertilizers with high inputs for providing food security and matching the overpopulation.