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317264

Phenotypic Characterization, Genetic Variation and Yield Performance of Some Egyptian Barley (Hordeum vulgare L.) Genotypes

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

Plant biodiversity

Abstract

       Seventeen promising lines of barley (Hordeum vulgare L.) were grown in two consecutive growing seasons to evaluate the morphological, genetic variation and yield performance under field experimental conditions compared with three varieties ( Giza 2000, Giza 132 and Giza 133). Twenty genotypes were distributed in a randomized complete blocks design in three replications. Twenty nine morphological traits were described using UPOV (The International Union for the Protection of New Varieties of Plant) Guidelines. Results showed that most of the morphological and yield characters were very different among all genotypes. Meanwhile, the heading data and maturity date characters varied among the lines comparing to their check varieties. The seed yield components had the highest mean values in lines 1 to 7, as the seed index had the lowest weight in two lines and reached the superior weight in 10 lines. The molecular characterization was carried out using 15 anchored-ISSR primers. The selected ISSR primers amplified a reliable banding pattern with the studied barley genotypes. The total percentage of polymorphism was 84.5%, recorded for 240 polymorphic bands out of 284 total bands. The fifteen used ISSR primers were able to characterize 14 lines and 3 check varieties with total 35 unique markers. The highest genetic similarity was 0.84, and the lowest similarity index revealed 0.60. The resulted phylogenetic dendrogram clustered the twenty genotypes into two main clusters, where the check varieties grouped together with 0.75 similarity. The most noted observation was that the high level of genetic homogeneity and the high similarity matrix (ranged from 0.64 to 0.84 similarity) recorded for within and between genotypes. Based on the barley yield traits, out of ten revealed Eigen values, the first three PCs assigned 69.67% variation for all studied traits. These results concluded that 17 lines may be considered as genetic resources for indirect and direct selection criteria in breeding program to improve the grain barley quality and quantity.

DOI

10.21608/ijmae.2023.227195.1019

Keywords

Barley, Phenotyping, Agronomical traits, ISSR, PCA

Authors

First Name

Abeer

Last Name

A. Ahmed

MiddleName

-

Affiliation

Seed Technology Research Dept., Field Crops Research Inst., Agricultural Research Center (ARC), Giza. Egypt.

Email

abeerabdelaty2015@gmail.com

City

Giza

Orcid

https://orcid.org/00

First Name

Ahmed

Last Name

M. Attya

MiddleName

-

Affiliation

Barley Research Dept., Field Crops Research Inst., Agricultural Research Center (ARC), Giza. Egypt.

Email

-

City

-

Orcid

-

First Name

Shimaa

Last Name

Mostafa

MiddleName

-

Affiliation

National Gene Bank, Agricultural Research Center (ARC), Giza, Egypt, P.O. Box 12619, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

Amal A

Last Name

El-Mahdy

MiddleName

-

Affiliation

Seed Technology Research Dept., Field Crops Research Inst., Agricultural Research Center (ARC), Giza. Egypt.

Email

-

City

-

Orcid

-

First Name

Zeinab

Last Name

E. Ghareeb

MiddleName

-

Affiliation

Central Laboratory for design and Statistical Analysis Research, Agricultural Research Center (ARC), Giza, Egypt.

Email

ahh44831@gmail.com

City

-

Orcid

-

Volume

3

Article Issue

1

Related Issue

43537

Issue Date

2023-06-01

Receive Date

2023-08-04

Publish Date

2023-06-01

Page Start

1

Page End

28

Print ISSN

2974-4407

Online ISSN

2974-4415

Link

https://ijmae.journals.ekb.eg/article_317264.html

Detail API

https://ijmae.journals.ekb.eg/service?article_code=317264

Order

317,264

Type

Original Article

Type Code

2,762

Publication Type

Journal

Publication Title

International Journal of Modern Agriculture and Environment

Publication Link

https://ijmae.journals.ekb.eg/

MainTitle

Phenotypic Characterization, Genetic Variation and Yield Performance of Some Egyptian Barley (Hordeum vulgare L.) Genotypes

Details

Type

Article

Created At

18 Dec 2024