The 146S antigenic mass load is the key success of FMD vaccines.In the current study,
concentrated, ultra-filtered and quantified FMD 146S antigens were prepared for immunization of
cattle via S/C (sub-cutaneous) and mucosal routes. The immunological reactivity of the
prepared antigens was estimated by DOT-ELISA.The coupling of both concentration and
ultrafiltration methods, showed the highest 146S quantity peak and the strongest antigenantibody reaction in DOT-ELISA. The SNT results of the immunized cattle revealed that the
protective neutralizing antibody response was successfully elicited following single S/C
immunization.While, in cattle immunized via mucosal route, slight increase in antibody levels
was observed. In conclusion, this study gives an insight to the importance of preparing highly
purified and quantified 146S FMD antigens required for inducing potent immune response
especially in mucosal vaccines.