A total of 192 samples were collected from eight hatcheries allover Egypt (Giza, Menofia, Alex, Ismalia, Sharkia, Albhara, Kaluobia and Gharbea Governorates. Twenty-four samples were collected from each hatchery. The samples were examined for detection occurrence of E.coli and The antibiogram for recovered isolates. Our study showed that, the Occurrence of
E.coli in broilers environment reached 32.29%. with resistance against kanamycin, cephalexin, amikacin, cephaloridine, enrofloxacin and ampicillin. Moderate numbers (28-57%) of isolates were found to be resistant to ciprofloxacin, ceftiofur and tetracycline. Lesser percent (8.79-15.38%) of isolates were resistant to nalidixic acid, colistin and co-trimoxazoled, which
has negative impact on broilers production and also affect public health.