In the present study, 48 testicles and sera of apparently healthy male camels were collected
separately from El-Basateen abattoir, Cairo, Egypt. The testicles of different camels were
aseptically separated and examined microbiologically for the presence of Mycoplasma.
Mycoplasma species was isolated and identified biochemically from 10.41% (5/48) of the
total collected samples. Further confirmation of the isolates with molecular characterization
using PCR revealed 8.3% (4/48) of the collected samples were confirmed as Mycoplasma
bovis. Serological investigation using indirect ELISA against Mycoplasma bovis antigen
revealed a prevalence rate of 41.66% (20/48). ELISA found to be a rapid and applicable
technique while PCR was confirmatory and more specific for the detection of Mycoplasma
bovis infection in male camel genitalia.The minimum inhibitory concentration of antibacterial
agents revealed that Mycoplasma bovis isolates were most sensitive to Enrofloxacin and
Tylosin. The study focused the attention on the impact of Mycoplasma bovis infection as one
of the suspected causes of infertility and or reproductive disorders in male dromedary camels.