A total of 350 blood samples were collected from 104 cows, 169 ewes and 77 buffalo dams in 6 governorates in Egypt over a full one-year period (2016). Sera were tested for detection of Neospora caninum antibodies using commercial ELISA kit. From the 350 animals, 124 had a previous record of abortion. Antibodies to Neospora caninum were detected in 79 out of 350 serum samples among all animals in 6 governorates in Egypt with a percentage of 22.57%. Out of those 79 animals, seropositive cows were 34 (43.04%); ewes were 32 (40.51%) and buffaloes were 13 (16.46%). The Seroprevalence was 50, 36.76 and 13.33 % in aborted cows, ewes and buffaloes respectively. The prevalence of N. caninum antibodies was higher in the aborted females than in normal ones as it was 24.19 % in aborted females compared to 21.68 % in non-aborted females. . Regarding the geographical prevalence, the high incidence was (35.71%) in Beni-sweif Governorate followed by (25%) in Aswan; (20%) in Menia and Sohag; (16%) in Giza and lowest (10%) in Fayoum. Further studies to clarify the impact of neosporosis on the animal industry, losses due to clinical neosporosis in livestock in Egypt and application of control strategies for such disease in dairy farms is recommended.