This study carried out on 358 equine (horses, donkeys and mules) from El-Qalyubia, Cairo and Giza governorates (area of study) at end of their life cycle with critical injuries and in need to euthanasia. Questionnaire on methods of euthanasia was done during study with vets in non-government organizations (NGOs) and research centers from April 2015 to March
2016. All of these establishments in area of study used four different methods for euthanasia; first method by the intravenous rout of barbiturate overdose (Method A) represents a proportion of (9.5%) of total euthanized equine. Second method by used sedative e.g. xylazine then thiopentone 5-11mg/kg and lastly potassium chlorid 1-2 mmol/kg (Method B)
represents a proportion of (72.3%) of total euthanized equine. Third method occurred through secured equine and inhaled chloroform then bleeding was done by cutting of the common carotid artery until complete bleeding occurred (Method C) represents a proportion of (9.5%) of total euthanized equine. Fourth method were done by using sedative e.g. xylazine then
complete injection with magnesium sulphate saturated solution (Method D) represents a proportion of (8.6%) of total euthanized equine. Further studies were done to determine the effect of each method (A, B, C, D) on behavior, health include body condition score (BCS) and welfare of euthanized equine.