Avian coccidiosis is one of the most important diseases affecting the intensive poultry industry worldwide. Coccidia are almost universally found wherever chickens are raised, and it is exceedingly rare to find a commercial chicken flock not affected. In our study, molecular based identification (conventional PCR) on positive fecal and intestinal samples for chicken
coccidiosis from broiler farms. PCR results revealed the prevalence of Eimeria tenella 100% then Eimeria necatrix 14.28 % from positive cecal and fecal samples, Eimeria acervulina 10.71% from positive intestinal and fecal samples. Our sequence revealed 98% identity with occivac -B E. tenella ITS-1 sequence.