In this study the immune response of pregnant cattle vaccinated with an experimentally prepared tissue culture live attenuated Lumpy Skin Disease virus (LSDV) vaccine was determined. Eight pregnant cows were vaccinated intradermal (I/D) with the field dose (103.0 TCID50/animal) of the prepared LSD vaccine, 2 pregnant cows were vaccinated I/D with 10x
of field dose of LSD vaccine (104.0 TCID50) for safety and 2 pregnant cows kept unvaccinated as control. All vaccinated and unvaccinated control pregnant cows were kept under clinical examination in insect proof rooms. Body temperature and post vaccinal reaction were recorded daily for 14 days. Vaccinated cows showed slight rise in body temperature started on
the 4th day post vaccination (PV) and returned to the normal temperature on the 9th day PV. Local reaction appeared at the inoculation site on the 4th day as a small size round firm nodule and persists for 12-15 days PV. Serum samples were collected from vaccinated cows showed protective level of LSD antibodies (1.5) and (1.32) for NI and ELISA SP ratio at the second week post vaccination and increased gradually till reached the maximum (3.25) NI and (2.35) S/P at 4th week post vaccination, respectively. SNT and ELISA carried out on collected serum samples from newly born calves born to vaccinated dams showed NI (2.25) and SP ratio (1.86) at the first week of age and persisted in protective level (1.5 NI and 1.11 S/P) until 6 months post parturition, then decreased and be lower than protective level. In conclusion, this study revealed that LSD live attenuated vaccine is highly immunogenic and safe as did not cause abortion nor LSD signs appeared in vaccinated pregnant cows, no teratogenic effect or congenital abnormalities in newborn calves and inducing a high level of antibody titer which gave protective maternal immunity persisted up to 6 months.