Bacteriological examination of faecal swabs collected from healthy (H) and diarrheic (D) calves, revealed isolation of 26 E. coli isolates from 56 calves with an incidence 46.4% Gram negative bacilli, non-sporulated. Then biochemical tests were done. Then serological test for representative number of samples from every farm identifying O1, O26, O44, O55, O115,
O119, O125, O146, O151 and 2 samples were untypable. The samples were sensitive to Norofloxacin with 80.7% and resistant to Ampicillin and Cefotaxime with 100%.The PCR for 4 gene ompA, stx1, stx2 and eaeA.