The objective of this study was to determine the effect of different levels of chicory leaf on performance and biochemical serum profile of broiler chicks. The feeding trial was carried out using 180, day-old Cobb broiler chicks. The birds were randomly assigned into three different groups; each group was subdivided into three replicates (20 birds each). The treatments were 1) served as control, and fed the basal diet only, 2) basal diet + 0.5% chicory leaves and 3) basal diet + 1% chicory leaves. At day thirty-five, three birds per replicate (pen) were randomly selected and slaughtered for some blood parameter measurements. The results showed that a significant improvement (P < 0.05) in body weight gain, (1871.38 ± 39.22, 1973.18 ± 26.37 and 1999.08±6.03) FCR, (1.90±0.013, 1.80 ±0.01 and 1.79±0.01) respectively with no significant difference in total feed consumption. In addition, blood indices showed a significant decrease (P ≤0.05) in liver enzymes activity as (ALT and AST),total cholesterol, triglycerides, LDL and VLDL with no significant difference in High density Lipoproteins (HDL) and kidney function indicators (serum uric acid and serum creatinine) between different inclusion rate of chicory (0.5% ,1%) and control. But total proteins and globulin fraction showed a significant increase in treated group with chicory by different levels compared to control with no significant difference in albumin fraction. It was concluded that chicory leaf can be used as an inexpensive, efficient and safe growth promoter and hepatoprotectant in broiler production.