The current study was performed to fill up the gap in knowledge of WSSV in the Egyptian shellfish diseases literatures. Randomly 268 different shellfish samples including 28 P. clarckii was collected from Abbassa, Sharkeya city and 240 marine samples (100 P. japonicus, 100 P. semisulcatus, 20 C. sapidus and 20 P. pelagicus) were collected from Suez-Gulf, Suez city and Murrah Lake, Ismailia city. Although, most of the examined shrimp (P. semisulcatus or P. Japonicus) from both Suez Gulf and Murrah Lake were apparently healthy .yet, 4 P. Japonicus and 1 P. semisulcatus from Suez Gulf presented typical white spot lesions on their carapace. On the other side, all shrimp samples from Murrah Lake were apparently healthy. Also almost all examined Crabs and crayfish samples were apparently healthy. Molecular detection using regular PCR has revealed that 2/10 pools of P. japonicus exoskeleton from Suez Gulf were positive for WSSV and 1/10 pools of the same species from Murrah Lake was also positive for the virus. The condition was little optimistic in case of P. semisulcatus only 1/10 pools from Suez Gulf was positive and pools from Murrah Lake were negative. Controversially, the freshwater Crayfish from Abbassa,
Sharkiya were all negative with the exception of only one sample out of 28. Moreover, Crabs from both Suez Gulf and Murrah Lakes were all negative for the virus supporting the well-known fact that Crabs are more immunologically competent than shrimp and crayfish.