Al-Dakhla region wells water contains high levels of some heavy metals including; cadmium (0.15 ppm ±0.006), lead (0.29 ppm ±0.011) and iron (0.86 ppm ±0.019) than that of tap water, WHO, (2011) and Egyptian standards 1589/ (2005) limits of drinking water. Such high levels of these heavy metals induced adverse deleterious effects in cows including increased serum concentration of lead (Pb), cadmium (Cd) and potassium (K), malondialdehyde (MDA), urea, creatinine, activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ-glutamyle aminotransferase (γGT) enzymes with decreasing of superoxide dismutase (SOD) enzyme activity, reduced glutathione (GSH), total protein, albumin, albumin/globulin ratio (A/G%), iron (Fe) zinc (Zn), calcium (Ca), phosphorus (P), magnesium (Mg) and sodium (Na)
concentrations.