Helicobacteriosis is worldwide infection caused by Helicobacter species. It affected both human and animals. The study investigate Helicobacter species in companion animals (Dogs andCats) and its zoonotic and public health repertoire.Samples were collected from apparently healthy companion animals (70 dogs and 65 cats) and 70 human patients in contact with the companion animal from Cairo and Giza governorates. The samples included serum samples,
feaces and stool samples and biopsies ofgastric fundus fragments5mm approximately.All samples examined by culture,biochemical, serology and molecular identification. The study reveals 43.4% Helicobacter species by PCR. H. heilmannii is more predominant with ratio 16% and H.pylori reveals 6%,while all samples were negative for H. felis. In conclusion, dogs and cats are reservoir and play a major transmission route for human helicobacters infection.