Bovine tuberculosis is a chronic and contagious disease that affects domestic animals, wildlife, and humans. Caused by Mycobacterium bovis, Bovine tuberculosis causes major economic losses and poses a serious constraint to international livestock trade. In Egypt, although there is a national Bovine tuberculosis control program, epidemiological studies are crucial to identifying the source of bovine tuberculosis infection, and its transmission dynamics and host preference. This article considers a trial to give first epidemiological snap shot about Bovine tuberculosis situation in Egypt. By examining 100 samples collected from slaughterhouse surveillance studies conducted in 2016 in El-Bassatin slaughter house, Cairo and apply epidemiological tool (spoligotyping) in all DNA extract after culturing, all positive samples on culture and biochemical reactions give negative sample with spoligotyping approach