Fifteen Egyptian buffalo calves with an average live body weight (LBW) 212.27 ± 8.84 kg and 13.0 + 0.42 months old were used for 6 months experimental period and randomly divided into three treatments (5 calves per treatment) to study the effect of drenching propylene glycol and calcium propionate as glycogenic precursor's supplementation during
growth period on nutrient digestibility and selected blood parameters. 1st group drenched three liters of saline solution (Nacl 0.9%) without addition was served as control, the 2nd group (PG) drenched 300mL of propylene glycol dissolved in three liters of (Nacl 0.9%) and the 3rd group (Ca-Pr) drenched with 335g of calcium propionate dissolved in 3 liters of (Nacl 0.9%). Results showed that daily dry matter intake decreased (P>0.05) in PG and Ca-Pr drenched buffalo calves than control group. Also,DM, OM,NDF and EE digestibility was not influenced by treatments during the metabolism trial. However, CP and CF digestibility tended to be higher in Ca-Pr treatment (P<0.05) and slightly improvement nutritive value as
(TDN and DCP). The overall means of plasma total protein, globulin, ALT, AST/ALT ratio, plasma urea, creatinine, triglycerides and cholesterol concentration remained un-affected among treatments however, the albumin and albumin/globulin ratio of Ca-Pr and PG groups were significantly higher (P<0.05) than control group. AST plasma activity was significantly lower (P<0.05) in PG and Ca-Pr experimental groups. Moreover, red blood cells count (RBC) and packed cell volume was decreased in PG treatment. Hemoglobin content, WBC count and balance plasma phosphorous, sodium and potassium concentration showed no change in all treatments.While,plasma calcium increased (P>0.05) in Ca-Pr calves (10.70 mg/dL) compared to PG and control groups (9.73 and 10.08mg/dL) respectively. PG and Ca-Pr showed higher significant difference than the control group on T3 (115.32, 123.07 and 100.71 ng/dI) and T4
(4.03, 3.81 and 3.44 ug/dl) respectively. It is worthy to mention that previous findings suggested a potential influence of PG and Ca-Pr which had widely safe accepted as a new energy source and calcium additive in buffalo calf's growth diet