The Chlamydiae are a diverse group of obligate intracellular Gram-negative bacteria that are known to infect a wide variety of host species. The present study was carried out on 3 species of farm animals (cattle, sheep and goat). The samples were collected from 2 groups of animal. The first group was apparently healthy animals for screening antibodies and antigen against Chlamydia abortus and Chlamydia pecorum for surveillance study. The second group was animals showing different clinical findings such as respiratory disorders, keratoconjunctivitis, vaginitis, abortion and diarrhea. The samples that collected from cattle, sheep and goats were 40 fecal samples, 30 nasal swabs, 3 ocular swabs, 18 vaginal swabs, 10 samples from internal organs and 65 serum samples. Diagnosis was based on complement fixation test (CFT) for detection Chlamydial antigen in feaces, nasal, ocular and vaginal samples. In addition, it depended on dedication of chlamydial antibodies in serum samples of farm animals (cattle, sheep and goat). In serum samples positive results for antisera of Chlamydia spp. were 69 %, 100 % and 78%, respectively. Regarding fecal samples, positive results for antigen of Chlamydia spp. were 100%, 55 % and 88%, respectively. Regarding vaginal swabs, positive results for antigen of Chlamydia spp. were 75 % in sheep and 67 % in goats. Meanwhile nasal swabs showed positive results for antigen of Chlamydia spp. were 86% for cattle and 69% for sheep and in ocular swabs, positive results for antigen of Chlamydia spp. were 100% for sheep. Chlamydia was isolated on embryonated chicken eggs (ECE) and the yolk sac was stained with Gimenez stain. The results of fecal samples of cattle, sheep and goats revealed the positive results for presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum were 100%, 89% and 75%, respectively. Regarding vaginal swabs, positive results for presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum were 100% in sheep and 92% in goats. Regarding nasal swabs, positive results for presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorumwere 78% and 87% for cattle and sheep. Regarding ocular swabs, positive results for presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum were 100% in sheep. Finally the percentage of Chlamydia abortus and Chlamydia pecorum in cattle 68.5%, 31.5%, respectively, in sheep 66%, 34%, respectively and in goats 55%, 45%, respectively. These results were confirmed through staining six random positive samples by toluidine blue stain as a part of procedure of Transmission electron microscope (TEM)). The collected internal organs from animals were stained by Giemsa stain and the positive results included presence of inclusion bodies of Chlamydia abortus, and Chlamydia pecorum were in 63% in cattle and 50 % in goats. The present study confirmed the work by indirect immunofluorescence test and the positive results for presence of inclusion bodies of Chlamydia spp. in 53% of cattle, 60% of sheep and 46% in goats. Transmission Electron Microscope (TEM) confirmed such results also on six random positive samples to confirm the presence of inclusion bodies of Chlamydia abortus and Chlamydia pecorum in infected yolk sac. The results suggest that, the farm animals (cattle, sheep and goat) may be reservoir of Chlamydia abortus and Chlamydia pecorum and thus shed the organism from natural orifices