Three hundred forty-five swaps were collected from different sources of suspected bacterial contamination sites in cattle slaughter hall in El-Monieb slaughterhouse in Giza governorate, Egypt.Those samples were processed aiming for isolation and identification of E. faecalis. The results revealed that out of 345 samples, 122 E. faecalis samples were recorded
representing 35.4% of the samples. The mean count E. faecalis among the different sources was 5.73 ± 1.7-log10 CFU/ cm2. The sensitivity test for 16 different antimicrobials showed that all the 122 E. faecalis samples were multidrug resistance. The electrophoretic profile of PCR for vanA gene was confirming vancomycin resistant E. faecalis isolates in 26 samples
(21.3%). It was concluded that, they are more than one source of Enterococci contamination before, during and after slaughtering process that could act as a public health risk and play a potential role in food safety issue.