Staphylococcus aureus and Methicillin resistant Staphylococcus aureus (MRSA) are widely spread in companion animals. The study was to investigate the Prevalence of MRSA in both nasal and wound swabs from pet dogs by conventional method and molecular characterization for detection of mecA gene using specific primers to confirm MRSA. A total of 101 pet dog
samples (67nasal swabs and 34 wound swabs) were collected from 101 pet dogs. All samples were cultured for the isolation of Staphylococcus aureus using selective media and biochemical tests. Isolates were identified as MRSA after antimicrobial susceptibility testing. PCR for mecA gene was applied. Nineteen nasal MRSA isolates were recovered from 67 (28%) nasal swabs and 6 wound isolates from 34 (17.6%). All isolates were resistant to cefoxitin and sensitive to both polymyxin B and vancomycin. All the 25 MRSA isolates were positive to mecA gene. The aim of this study is to Investigate Methicillin-resistant Staphylococcus aureus (MRSA) through antibiogram using Cefoxitin disc and molecular detection of mecA gene in pet dogs. The result of this study indicated the possible role of pet dogs in the epidemiology of MRSA zoonosis in the community to be considered as an emerging veterinary pathogen of pets with a public health burden.