Necrotic enteritis is a great problem in poultry industry globally. Little information exists concerning the pathogenesis of necrotic enteritis in turkeys. Bacteriological and parasitological examination of 100 intestinal samples revealed that 55%, 39% and 34% had C. perfringens, Eimeria species and mixed infection, respectively. Investigation of toxigenic subtypes among the isolates by multiplex PCR showed that (53.8%) of studied isolates considered as C. perfringens type A as harbored cpa gene only. Antimicrobial susceptibility results demonstrated that, the highest resistance against colistin (89%). Amoxicillin was most effective against 45 (81.8%) tested isolates of C. perfringens. Therefore, the present work was designed to develop an experimental model of necrotic enteritis and coccidiosis in turkey to study the growth performance, biochemical parameters and histopathological changes associated with it. Seventy, one day old turkeys were divided into seven equally groups, group (1) negative control. Group (2, 3 and 4) was infected orally with Eimeria oocysts, C. perfringens and both Eimeria oocysts and C. perfringens, respectively. Group (5) was treated with diclazuril after infection with oocysts of Eimeria. Group (6) was treated with amoxicillin post infectionwith C.perfringens.Group (7) was treated with (diclazuril+amoxicillin) after infection with both Eimeria oocysts + C. perfringens. Experimental study revealed disturbance in proteingram, lipogram and liver and kidney function test detected throw the biochemical and pathological changes. The amoxicillin and diclazuril had significant improvement in growth performance, in addition to reduction in mortalities lowered number of oocysts, reduce the severities of necrotic enteritis (NE) and clostridia count in addition to amelioration of the biochemical and pathological changes.