Herbal extracts have been reported to modulate immune response to combat immune-related diseases .The present study investigated the effect of Echincae purparea extract ; Orguinum vulgaris extract and mix both of them on serum total protein , albumin and globulin concentrations in rats as animal model .Eighty adult male rats were assigned into four groups(20 rats in each one).The 1st group kept as control ,the 2nd group was given Echincaepurparea extract in a dose of 300mg/day/rat, the 3rd group was given Orguinum vulgaris extract in a dose of 200mg/day/rat , while the 4th groups was given mixture of both extracts in a dose of 100mg/day/bw/rat of Orguinum vulgaris and 150mg/day/rat of Echincae purparea extract. All herbal extracts were given in drinking water for 15 days .Blood samples were collected from rats on the 1,3,5,7,9,12,15 days following the first day of adminstertion through medial canthus vein of eye, the serum total prorien was increased non significantly ( 6.20+/-0.2a) in rats treated by mixure of extracts in the fifteenth day post treatment as compared with control group ( 5.9+/-0.100a) Serum albumin was significantly increased in the 7th and 9th days post treatment with Echniaca extract compared with Organium Vulgaris extract also there was significant decrease in the 15th day post treament with Oragiumn Vulgaris with Echincae purparea extract and mixure was not significant. Gama globulin was significantly increased in the 15th day Post treatment with mix of Echniaca extract and Organium Vulgaris extract compared with each extract treatment alone