Nowadays, infectious diarrhea in dogs and cats is a frequent disease problem with pet animal veterinarians. The present study discussed the occurrence of some entero-pathogenic pathogens such as E.coli, Klebseilla in fecal samples of dogs and cats with the detection of virulence genes fecal samples were collected from 180 diarrheic dogs and 70 diarrheic were
submitted to the 6th of October Veterinary.Enteropathogenic E.coli isolates were recovered from 42% (n=75/180) and 40% (n=28/70) of dog and cat samples, respectively. On the other hand, K. pneumoniae incidence in dogs was 21% (n=37/180) and 13% (n=9/70) in cats.
Antibiogram of the examined isolates revealed that 68% of dog isolates and 79% of cat isolates of E. coli highly resistant to ampicillin/sulbactam. The resistance to ampicillin and augmentin was conferred by (59%,64%) and (49%54%) of dog and cat isolates, respectively. Meanwhile, K. Pneumoniae isolates from dogs and cats were highly resistant to ceftazidime (100%,100%), ampicillin (78% and 88%), amikin ( 78% and 67%), augmentin (62% and 67%) respectively. The molecular characterization of the isolates proved high frequency of the virulence genes VT2e, eaeA, LT, STa, Cnf1, mrkA, ecpA, fimA and fimH. In conclusion, it looks like that, the virulence genes detected in the isolates were linked with diarrhea in both types of tested pets at various ages. Potential active transmission of such types of bacteria to humans is expected; thus it represents an escalating potential public health.