Abstract
    
     This research aimed to explore the impact of different levels of dietary Sargassum muticum algae and Pulicaria undulata (Rabol) extract on various aspects including performance, immune response, blood parameters, as well as manure and ammonia emissions in broiler chickens. A sum of 315 one-day-olds, the average weight of the bodyis (37.37 ± 0.38) grams, were utilized for the study. These were divided into five groups, each receiving different feed additives incorporated daily into their diets. Growth performance, blood parameters, manure characteristics, and ammonia emissions were carefully monitored and analyzed. The results indicated that the group receiving the T5 diet exhibited the highest total feed intake (2493.40 g) and total gain (TG). Conversely, the group fed the T6 diet showed the lowest feed intake (3938.12 g), followed by the T7 group (4014.41 g). Additionally, the T5, T6, and T7 groups demonstrated superior total feed conversion ratios (TFCR), while the T7 group recorded the highest performance index (PI) and total performance index (TPI%) at 162.13% and 167.12%, respectively. Regarding manure characteristics, the T7 group showed the best results in terms of manure ash content, nitrogen levels, and pH values, with values of 22.03, 1.72, and 5.4, respectively. Furthermore, the T7 group exhibited significantly lower levels of manure ammonia emissions compared to the other groups (p<0.05).Overall, dietary supplementation with 0.3% Pulicaria undulata extract per kilogram of feed (T5), or a combination of 1.0% Sargassum powder (100 g/kg feed) and 0.3% Pulicaria undulata extract per kilogram of feed (T6), or 1.5% Sargassum powder (150 g/kg feed) and 0.3% Pulicaria undulata extract per kilogram of feed (T7), may contribute to protein protection from free radicals and potentially reduce protein consumption in immune globulin synthesis, thus benefiting broiler chicken health and performance.