The effect of irrigation with treated wastewater on soil physical, chemical and biological properties was evaluated in this study. Therefore, soil samples were collected from three sites different dimensions and directions of the Ismailia Canal, and the chemical, physical and biological properties of the collected soil samples were determined, and various soil analyzes were performed, such as pH, electrical conductivity, and macro and micro elements. Dehydrogenase activity was determined. Results showed that the use of treated irrigation water led to a slight increase in pH, EC, as well as macro and microelements. In addition, led to a decrease in microbial activity expressed by the dehydrogenase enzymes activity, compared to other sectors.