ABSTRACT
The purpose of the current investigation was to determine the impact of adding various
oncentrations of glycine betaine (GB) to spermatozoa from camels that had been extended with a lactose-yolk-citrate (LYC) extender and kept at 5 OC for three days. Using an artificial vagina, semen was extracted from five camels and prolonged with LYC extender free-GB medium (first medium), or at 100 mM, 200 mM, or 300 mM GB for second, third, and fourth media, respectively. The ultimate sperm concentration was 100x106 sperms per ml, and she camels that were inseminated using extended semen from first, second, third and fourth groups were determined after 45 days. The percentage of motile camel spermatozoa extended with LYC extender added to 100 or 200 mM GB were considerably greater (P 0.05) compared to 300 mM GB or free-GB media, according to the results. However, upon storage time, the proportion of dead, aberrant, damaged acrosomes and chromatin as well as the activity of the AST and ALT enzymes were considerably (P 0.05) lower than before. With or without the GB medium, the quality of the camel semen considerably (P 0.05) decreased when storage time was extended. Additionally, the first, second, third, and fourth media's respective fertility rates for one day of the artificially inseminated dromedary she-camels were 31.57, 52.63, 47.61, and 26.31%. In conclusion, GB addition to the cooled camel spermatozoa at 100 or 200 mM, while being stored at 5 OC, was found to enrich the camel sperm quality and fertility rates.