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The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

• Histology

Abstract

Background: Dutasteride is an anti-androgen used in the treatment of prostatic diseases. Pumpkin seed oil (PSO) is a functional food oil with an antioxidant and immunomodulatory actions. Aim: assessing the possible protective effect of PSO on dutasteride-induced changes on spleen of adult male albino rats via histological and immunohistochemical methods. Methods: 30 male albino rats 200-250 gm divided into; Control group, Dutasteride low dose (DLD): rats received dutasteride 0.05 ml /day, Dutasteride high dose (DHD): rats received dutasteride 0.15 ml/ day, DLD + PSO: dutasteride low dose group treated with 1.5 ml/kg/day of PSO, DHD + PSO: dutasteride high dose treated with 1.5 ml/kg/day of PSO. Doses were orally for 42 days. Results: DLD &DHD groups (Groups II &III) showed marked histopathological changes dose dependently. There was dilated blood sinusoids in the subcapsular areas & throughout the parenchyma, disorganization, dilatation& expansion of the red pulp with reduced splenic cords, disorganization & reduction in the size of the white pulp lymphoid follicles having lymphocytes with pyknotic nuclei and vacuolated cytoplasm, macrophages containing apoptotic bodies, dilated central arteriole with detached endothelium and vacuolated wall, and significantly increased collagen mean area%. Also, significantly increased mean number of immune-positive cells for CD68& TGF-β while decreased for CD3 immunohistochemical expression. Regarding the DLD+PSO& DHD+PSO groups (Groups III& IV); marked amelioration of the previously mentioned results was observed. Conclusion: PSO has an ameliorative effect on the histopathological changes on rat spleen induced by dutasteride. So, PSO is beneficial in the treatment of side effects of dutasteride.

DOI

10.21608/ejctr.2024.286991.1016

Keywords

spleen, Dutasteride, Pumpkin seed oil, histopathology

Authors

First Name

Samah

Last Name

Kandeel

MiddleName

-

Affiliation

Professor of Histology and Cell Biology Faculty of Medicine, Tanta University

Email

samah.kandeel@med.tanta.edu.eg

City

Tanta; El-Gharbeya

Orcid

0000-0003-4559-9005

First Name

Remon

Last Name

Remon S. Estfanous

MiddleName

-

Affiliation

Anatomy and Embryology department, Faculty of Medicine, Tanta University, Egypt..

Email

sskandeel@hotmail.com

City

-

Orcid

0000-0003-4559-9005

First Name

Shimaa

Last Name

Badr

MiddleName

Mahmoud

Affiliation

Histology &cell biology Department _Faculty of Medicine _Tanta University

Email

shaimaa.badr@med.tanta.edu.eg

City

-

Orcid

0000-0001-5161-1096

Volume

2

Article Issue

2

Related Issue

48770

Issue Date

2024-06-01

Receive Date

2024-05-03

Publish Date

2024-06-01

Page Start

52

Page End

81

Print ISSN

2812-5436

Online ISSN

2812-5444

Link

https://ejctr.journals.ekb.eg/article_363300.html

Detail API

https://ejctr.journals.ekb.eg/service?article_code=363300

Order

363,300

Type

Original articles

Type Code

2,629

Publication Type

Journal

Publication Title

Egyptian Journal of Cell and Tissue Research

Publication Link

https://ejctr.journals.ekb.eg/

MainTitle

The possible protective effect of pumpkin seed oil on dutasteride-induced changes on spleen of adult male albino rats: Histological and immunohistochemical study

Details

Type

Article

Created At

18 Dec 2024