The goal of this study is to evaluate the efficacy of bone marrow derived mesenchymal stem cells (BMMSCs) in the prevention of fibrosis following induction of CRS type 3 in rats, and in addition, to assess their effect on FGF23 and Klotho genes, renal functions, and histopathological features. A total of eighteen male albino rats were enrolled in this study divided equally into 3 groups; group I: healthy control, group II: CRS without treatment, group III: CRS treated with BM-MSCs. Rats were
used to generate Cardiac Hypertrophy (in vivo), by ligating their right renal artery, and group III was injected with stem cells after the insult. Serum levels of urea and creatinine were assessed, FGF23 and Klotho genes expression in cardiac and renal tissue by quantitative real time PCR were evaluated. Histopathological examination of tissues was performed. Results revealed that serum levels of urea and creatinine were elevated with significant increase in FGF23 and decrease in KLOTHO levels in diseased group compared to control and treated group. In treated group with BMMSCs, FGF23 was markedly inhibited, and KLOTHO level was upregulated with little
significant difference between treated group and normal control. In addition, there was decrease in urea and creatinine levels. Histopathological examination of treated group revealed improvement of induced fibrosis in renal and cardiac tissues compared to diseased group. The data suggests that
MSCs might have therapeutic effect on cardiorenal syndrome type 3.