The goal of this study is to investigate the role of the fibroblast growth factor 23 (FGF23) / Klotho (Klotho) molecular signaling axis in an experimental model of cardiorenal syndrome type 3 resulting from right renal artery ligation leading to renal reperfusion ischemia. The study used 12 male Wistar rats (6-8 months old, weighing 230-300g) split into 2 groups of 6 rats each (Group I, controls) and Group II, diseases, where Cardiac Hypertrophy was created as a result of renal ischemia by ligating the right renal artery. Gene expression of FGF23 and Klotho in cardiac and renal tissue was measured using quantitative real-time PCR, and blood urea and creatinine levels were measured clinically.All afflicted rats had increased amounts of creatinine in their blood, and histological analysis revealed renal ischemia and heart hypertrophy. According to real time PCR, FGF23 expression has dramatically increased in cardiac and renal tissues whereas alpha klotho expression has decreased.