Background: Recent years increasing antibiotic resistance, the possibility of novel plant derived antibiotics is very important as alternative compounds for antibiotics. Plant extracts involves antibacterial properties.  Objective: The aim of the current study was to evaluate the efficacy of 3 essential oils extracted from 3 plants against pathogenic bacterial isolated from wounds and burns. Materials and methods: The essential oils of A. citrodora, R. officinalis and T. vulgaris were extracted and prepared in serial dilution concentrations. A total of 150 bacterial isolates from wounds and burns were isolated and identified. Results: The results showed that Pseudomonas was the most frequent as 51 (34%) isolates, while Enterococcus and Rhizopium were the least as 1 (0.67%) isolate.  A susceptibility test was conducted by using 15 different antibiotic discs. Trimethoprim was the most resistant antibiotic for bacterial isolates (79.33%), while Imipenem was the least (40%). Moreover, the susceptibility test was revealed the most resistant was Rhizopium with 86.67% and Enterococcus was least with 20%. The highest antibiotic resistant isolates 22 were selected for determining the synergistic effect of volatile oils that inhibited of bacterial growth, the concentration of significant synergistic effect was 156.25, 156.25 and 39.0625 ppm of A. citrodora, R. officinalis and T. vulgaris respectively. Conclusions: Little attention has been paid to evaluate the efficacy of Eos as alternative treatment instead of conventional remedy, thus this study tested the activity of 2 EOs against pathogenic bacteria.