Background: Remineralization of early enamel lesions is considered one of the approaches of minimal invasive dentistry. Aim: This in vitro study was conducted to evaluate the remineralizing effect of green tea, stevia extract, and fluoride-based mouthwashes on incipient enamel lesions using a laser fluorescence device (Diagnodent). Materials and Methods: Sixty human extracted premolars were randomly distributed into six groups of 10 teeth according to the remineralizing agent used. Group I: 0.5% stevia aqueous solution; Group II: 5% stevia aqueous solution; Group III: 0.5% green tea aqueous solution; Group IV: 5% green tea aqueous solution; Group V: Fluoride mouthwash (positive control group) and Group VI: artificial saliva (negative control group). Laser fluorescence testing (Diagnodent) was used for baseline assessment, after 48 hours, demineralization, and after 1 week of the remineralization phase. Results: After 7 days of remineralization, the negative control group showed the highest laser fluorescence mean value while all experimental and positive control groups showed the lowest laser fluorescence mean values. 5% stevia and 0.05 % Green tea mouthwashes showed an insignificant difference between baseline and remineralization. Conclusions: Stevia and green tea mouthwashes had a positive impact on incipient enamel remineralization.