Acrylamide was well known to induce reproductive toxicity with an impact on reproductive functions on both male and female. The current investigation was designed as a trial to assess any changes in ovarian hormones and histology brought on by the administration of acrylamide for three weeks. Methods: Animals were split into two groups (N = 10) and fed a healthy diet. and DMSO (1%) saline (9%), Acrylamide (AA) group received acrylamide in saline (9%), (20 mg/kg b. w.) for 21days. Alteration in the level of ovarian hormones were assayed and correlated with histological changes in ovarian tissue. Results: Estradiol levels in the serum were considerably increased after acrylamide administration, but progesterone levels were significantly decreased. Histological study revealed that AA causes cyst formation, corpus luteum d generation which pointed to ovarian follicular regression. In conclusion: Results showed that AA modulated hormonal levels in female rats that related to alteration in ovarian structure