Background: Perimenopause represents a critical life stage encompassing a range of physiological and psychosocial changes that require adaptions to optimize health. The present study aimed to Evaluate the effect of self-care guidelines on the quality of life among perimenopausal women. Design: Quasi- experimental (pre- posttest). Setting: Administrative buildings of Suez Canal University in Ismailia city. Sample: Purposive sampling of perimenopausal women employed in Suez Canal University and having perimenopausal symptoms was recruited in study. The selected women were randomly allocated into two groups: Study and control group (102 women each).Tools of data collection : A structure interview questionnaire, menopausal rating scale, self-care diary and quality of life assessment questionnaire.Results: There were a statistically significant increase in total knowledge and self-care practices for women in the study group compared to women in control group post intervention and during follow up (after 3 months) (P = 0.01, 0.02). There were statistically significant increases in total quality of life in the study group compared to women in the control group post-intervention & after 3 months. (P = 0.004, 0.01). The study concluded that: Guidelines is an effective complementary health approach for improving the quality of life in perimenopausal women. Recommendation: Increase level of awareness among women regarding issues of menopausal symptoms. Self-care guidelines should be used in health care centers as a routine care for perimenopausal women.