There have been always concerns about the effect of sugar intake on health, especially in indviduals suffering from diabetes. Attempts have been made to replace sugar with healthy and safe sweeteners. Owing to its low- calorie content, stevia has attracted much attention as a sugar replacement. The aim of this study was to examine the effects of two sweeteners namely; stevia and sucrose on blood glucose levels in healthy individuals. It has been established to help reduce blood glucose levels. The influence of sucrose and stevia on healthy individuals (n=33, aged 12-55, body mass index 24.4±  8 kg/m2) was studied. Participants were allowed to oral consumption of 100 mL of three beverages containing 0.2 mg/mL of surcrose, 5 mg/mL of stevia, or 10 mg/mL  of stevia on different experimental days. The results showed a significant difference a glycemic response on days, when sucrose and stevia were consumed, while no siginificant differences were recoreded between the two doses of stevia. Based on our findings, it could be concluded that the consumption of stevia in a short time period does not raise blood glucose in healthy individuals.