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238842

Genetic Analysis of Fluoroquinolone Resistant Genes in Mycoplasma gallisepticum Field Isolates

Article

Last updated: 29 Dec 2024

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Subjects

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Tags

Studies on Sequence Gene mutations and monitoring the causes of epidemic diseases

Abstract

Mycoplasma gallisepticum (MG) causes economic losses to poultry industry all over the world. This study aimed to investigate the emergence of fluoroquinolone (FQ) resistance in MG field isolates using sequence analysis of genes encoding the subunits of the drug's target enzymes. The results showed that two MG isolates were resistant to fluoroquinolones. Amino acid mutations were recorded in gyrA at codons 59, 73, 157 and 174, gyrB at codons 415 in addition to Glu at 416 and ParC at codons 92 and 167. No mutations were observed in gyrE. Two-dimensional analyses illustrated that mutations in the resistant genes; gyrA, gyrB, and ParC were associated with alterations in the predicted protein structures of quinolone-resistance determining regions (QRDRs). These mutations were accompanied by elevation of minimum inhibitory concentration (MIC) profiles to FQ. It was concluded that the mutations in genes encoding DNA gyrase enzyme were the important causes for the appearance of FQ resistance MG isolates. 

DOI

10.21608/ejceh.2019.238842

Keywords

Mycoplasma Gallisepticum, MIC, Antimicrobial resistant genes, Mutation, Two-dimensional analyses

Authors

First Name

Sahar

Last Name

E

MiddleName

O.

Affiliation

Mycoplasma Department, Animal Health Research Institute, Dokii, Giza

Email

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City

-

Orcid

-

First Name

Sally

Last Name

Abou-Khadra

MiddleName

H.

Affiliation

Microbiology Department, Animal Health Research Institute Zagazig branch.,Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Egypt

Email

-

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

33428

Issue Date

2019-11-01

Receive Date

2022-05-23

Publish Date

2019-11-01

Page Start

37

Page End

55

Online ISSN

2357-1039

Article File

EJCEH_Volume 5_Issue 2_Pages 37-55.pdf

PDF . 619.4kB

Link

https://ejceh.journals.ekb.eg/article_238842.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=238842

Order

238,842

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Genetic Analysis of Fluoroquinolone Resistant Genes in Mycoplasma gallisepticum Field Isolates

Details

Type

Article

Created At

23 Jan 2023