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254610

Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

Chemical Contaminants of food of animal origin

Abstract

The purpose of this research was to study the prevalence of enterococci and streptococci in milk and some dairy products. Survey included 50 samples of raw milk, 25 kareish cheese and 25 yoghurt samples which were collected from El-Behera governorate markets. The results revealed that the mean of chemical composition was 12.95±0.11, 86.34±0.26, 6.3±0.07 and 0.18±0.002 for total solid, Moisture content, pH values and titratable acidity, respectively. in raw milk samples; 36.8±0.87, 61.76±0.84, 3.87±0.11 and 2.27±0.037 in kareish cheese and 36.8±0.87, 76.48±0.94, 4.33±0.05 and 1.09±0.036 in yoghurt samples, Respectively. incidence of positive samples for aerobic plate count, streptococci and enterococci count were 88 , 26 and 22 % in raw milk samples, respectively . 76 , 36 and 36 % in kareish cheese and 56, 28 and 32 % in yoghurt samples, respectively. with mean values of 1.22x105 ±0.1x105, 4.5x103 ±0.7x103 and 6.3x103 ±1.9x103 cfu/ml in raw milk samples; 8.9x104 ±0.76x104 , 4.7x103 ±0.87x103  and 5.7x103 ±1.6x103 cfu/gm in kareish cheese samples and in yoghurt samples were 7.6x104 ±0.59x104 , 3.7x103 ±0.6x103 and 5.5x103 ±0.64x103 , respectively. All isolated Enterococcus faecium, Enterococcus faecalis, S. agalactiae and S. dysgalatiae were confirmed at a genus level using specific primer targeting 16s rRNA gene in S. dysgalatiae and Enter. Faecalis; sod A gene in enterococcus faecium and cfb gene in S. agalactiae. Desired amplicon for virulence genes were obtained.

DOI

10.21608/ejceh.2016.254610

Keywords

Milk, Dairy products, enterococci, Streptococci, PCR, 16S rDNA

Authors

First Name

Seham

Last Name

Gorgy

MiddleName

F.

Affiliation

Animal Health Research Institute

Email

-

City

-

Orcid

-

First Name

El Asuoty

Last Name

S.

MiddleName

M.

Affiliation

Animal Health Research Institute, Damanhour branch

Email

-

City

-

Orcid

-

First Name

Saber

Last Name

S.

MiddleName

A.

Affiliation

Animal Health Research Institute, Damanhour branch

Email

-

City

-

Orcid

-

First Name

Abeer

Last Name

Ali

MiddleName

H.

Affiliation

Alexandria branch AHRI, Egypt

Email

-

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

33292

Issue Date

2016-11-01

Receive Date

2022-08-14

Publish Date

2016-11-01

Page Start

500

Page End

515

Online ISSN

2357-1039

Link

https://ejceh.journals.ekb.eg/article_254610.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=254610

Order

254,610

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Prevalence of Enterococci and Streptococci in Raw Milk and Some Dairy Products and The Subsequent Alteration on Quality

Details

Type

Article

Created At

23 Jan 2023