Acetamiprid (ACP) is neonicotinoid insecticide and it is the most highly effective insecticides worldwide for crop protection and control fleas infesting in livestock and pet animals. It has potential toxicity to mammals and there is no clear data regards to hepatotoxicity, nephrotoxicity and oxidative stress at low doses. The present study aimed to investigate the acute toxicity of ACP as formulation toward male adult albino rats and evaluate the impact effect of repeated sub-lethal dose of ACP. The high dose equal 1/10 of estimated LD50 and the low dose approximately equivalent 1.5X of No Observed Adversed Effect Level (NOAEL) dose. The study concluded the examination of hepatotoxicity, nephrotoxicity and some parameters that covered oxidative stress. The results revealed that, ACP may not have such extensive effects as inducer to oxidative stress but may be induce synergism of the defense system to increase the antioxidant self-system. Moreover, the histopathological investigation revealed that the exposure to high dose of ACP led to destructive and severe effects on the kidney tissue but may be has moderate effects on the liver tissue. The low dose which equal approximately 1.5X of NOAEL led to slight effects on the liver tissue while the kidney tissue was still more affected by this insecticide. Finally, It seems that the ACP induces the adverse effect at the high dose level but the low dose level and short term exposure may not make adverse effect. So, it can be recommend to use this insecticide as long as using the recommended dose.