The present study aimed to investigate clinico-pathological changes on turkey poult infected with Pasteurella Multocida (P. Multocida), about 200 turkey poults, 15th day old, were subjected for bacteriological examination to obtain 80 healthy turkey poults free from any bacterial infection. Turkey poult were divided to 4 equal group, 1st Gp healthy poults non treated (control), 2 nd Gp healthy poults injected with 20 mg/kg bwt. spectinomycin for 5 days, 3 rd Gp infected non treated turkey poults and 4 thGp infected poults treated with 20 mg/kg b. wt spectinomycin for 5 days. Clinical signs, mortality rate and post mortom examination were recorded. Effect of P. Multocida and spictinomycin on body weight was studied. At the end of treatment (1st day post treatment) 5 poults from each group were sacrificed, blood samples were taken for hemato-biochemical analysis. Samples from liver and kidney for detection of spectinomycin residue, beside samples from internal organs were taken for pathological study. Turkey poults received spectinomycin showed significant increase in weight gain, improve feed conversion rate, significant decrease in RBCs, Hb, PCV% and insignificant effect in leukogram, total protein, albumin, globulin, AST , ALT, ALP, uric acid, creatinine, serum total lipid, cholesterol and triglyceride as compared with healthy non treated group Pasteurellosis in turkey showed clinical signs of loss of appetite, depression, ruffled feathers, dropping of the wings, fever, diarrhea, poor growth, emaciation as well as cyanosis of comb and wattles and mortality rate was 25 %. Significant reduction in body weight, weight gain, RBCs, PCV%, Hb and significant elevation in feed conversion rate, WBCs, monocyte, globulin AST, ALT, ALP uric acid, creatinine and insignificant effect in heterophils, lymphocyts, Eosinophils, basophile, total lipid, cholesterol, triglyceride, total protein, albumin and A/G ratio . Spectinomycin residues were detected in liver and kidney up to 9 days post treatment but not detected at 12 days post treatment and its residues in kidney higher than in liver. The pathological examination of internal organs of different groups revealed, spectinomycin treated group (4) should mild pathological alteration in the internal organ,, however P. multocida infection induced severe pathological lesions as hepatic and lung tissue hemorrhage and congestion, spleen lymphocytic depletion, degenerative changes of renal tubules. cardiac muscle showed round cell infiltration , hemorrhage and degeneration of muscle fibers. The trachea showed mucosal desquamated epithelium and leukocytic infiltration but infected turkey poult treated with spectinomycin induced marked improvement in the pathological changes. It could be concluded that pasterullosis in turkey poult induced many reversible adverse effect on body performance and hemato-biochemical parameters which is confirmed by pathological changes. Spectinomycin in therapeutic dose is considerable effective in treatment of pasteurollosis in turkey poult