Administration of hormones to broiler chickens for performance-enhancing purposes may lead to deposit of residues in their carcasses. This could be a potential human health hazard upon exposure to these residues. Therefore, this study was carried out on ninety randomly collected fresh broiler samples to detect medroxyprogesterone acetate (MPA) and 17β- estradiol hormonal residues in muscle and fat of broiler carcasses by using Enzyme Linked Immun-Sorbant Assay (ELISA). The mean value of 17β-estradiol hormonal residue in muscle and fat of broiler carcasses were 0.090±0.005 and 0.182±0.010 ppb respectively. Sixty eight (75%) of muscle samples and thirty eight (42%) of fat samples were within the physiological limit (<0.05ppb). 17β-estradiol hormonal residue were within the permissible limit (1ppb) in all examined samples of muscle and fat. Medroxyprogesterone acetate residue (MPA) could not be detected in all examined samples. The public health significance and recommendation for the use of hormones were discussed