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247686

Some Bacteriological and Molecular Studies on ESCHERICHIA COLI as Causative agent of calves enteritis at Damietta Governorate

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Last updated: 05 Jan 2025

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Tags

Feed of pet animals and its diseases

Abstract

The present study carried out to evaluate the presence of diarrheagenic Escherichia as causes of diarrhea in young calves in Damietta governorate during period from November 2015 to March 2016. Bacteriological examination was done on a total of (100) fecal samples collected from young diarrhoeic calves of average age of 2 to 21 days to detect the prevalence of E. coli . The samples were grouped according to the age of the calves into 3 age groups as follows: 1st age group (2-7 dayes), 2nd age group (8-14 dayes) and 3rd group (15-21 dayes). The results revealed isolation of 27 strains of E. coli (27%). Serotyping of the isolated strains according to somatic (O) and flagellar (H) antigens detected the presence of 6 entertoxigenic E. coli (ETEC; 22.22%); 13 enterohemorrhagic E. coli (EHEC; 48.15%); 5 enteropathogenic E. coli (EPEC; 18.52%) and 3 enterinvasive E. coli (EIEC; 11.11%). Application of PCR for the detection of E. coli virulence genes including; two Shiga toxins: (Stx1; 88.89%), (Stx2; 51.85%), produced from Shiga toxin producing E. coli (STEC); intimin (eaeA; 29.63%) and hylA (59.26%) genes was done. Each isolate was found to carry one or more virulence genes. Mixed E. coli infection is strongly associated with the cases of diarrhea due to the presence of several virulence genes in some isolates. Diarrheic calves were found in high prevalence in the 1st age group (43%) whereas was found in prevalence in the 2nd group (33%) and in 3rd group (24%) .
Antibiogram study revealed that the isolated E. coli strains were highly sensitive to Ciprofloxacin and Cefotaxime, moderately sensitive to Gentamicin and resistance to Nalidixic acid and Erythromycine. The present study suggests the importance of maintain strict hygienic measures to prevent spreading any pathogens to healthy calves. 

DOI

10.21608/ejceh.2016.247686

Keywords

Diarrheagenic E. coli, calves, Virulence genes, Antibiogram

Authors

First Name

Rehab

Last Name

Dawod

MiddleName

E

Affiliation

Animal Health Research Institute, Damietta Branch, Egypt

Email

-

City

-

Orcid

-

First Name

Gihan

Last Name

Mohammed

MiddleName

M.O.

Affiliation

Animal Health Research Institute, Port-Said Branch, Egypt

Email

-

City

-

Orcid

-

First Name

Helal

Last Name

, I.M

MiddleName

-

Affiliation

Animal Health Research Institute, Port-Said Branch, Egypt

Email

-

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

33292

Issue Date

2016-11-01

Receive Date

2022-07-03

Publish Date

2016-11-01

Page Start

195

Page End

210

Online ISSN

2357-1039

Article File

EJCEH_Volume 2_Issue 2_Pages 195-210.pdf

PDF . 545.0kB

Link

https://ejceh.journals.ekb.eg/article_247686.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=247686

Order

247,686

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Some Bacteriological and Molecular Studies on ESCHERICHIA COLI as Causative agent of calves enteritis at Damietta Governorate

Details

Type

Article

Created At

23 Jan 2023