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247635

Molecular Characterization and Hemato Biochemical Studies of Reovirus in Ismailia Farms

Article

Last updated: 29 Dec 2024

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Subjects

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Tags

Studies on Sequence Gene mutations and monitoring the causes of epidemic diseases

Abstract

The avian reoviruse (AVR) induces various manifestations in chickens. They are associated with disease conditions including malabsorption syndrome and tenosynovitis. RT-PCR is a rapid, sensitive and specific diagnostic test for ARV detection and avoiding economic losses. Three broiler poultry farms in Ismailia with reovirus like symptoms were screened for Reoviruses in SPF embryonated chicken eggs. In these farms, there were 20-25% morbidities at 24th day of age and 3-4% morbidities at 14th day of age, and 5-6% mortalities at 24th day, while 2-3 % is at the 14th day. Three isolates of Reovirus were obtained and characterized and identified by RT-PCR. Electrophoretic pattern showed a specific band at 399 bp positive at lane 1, 3 and negative at lane 2. Results of haematological investigation showed low levels of total erythrocytic count (TEC), haemoglobin (Hb), and packed cell volume (PCV) but significant increase in level of total leukocytic count (TLC), absolute lymphocyte count (ALC) and absolute monocyte count (AMC) in infected chickens compared to apparently healthy. Biochmical estimation revealed marked elevation of total serum proteins, AST; ALT. Pathological changes in internal organs of the infected chicks were also described. The proventriculus revealed marked hyperplasia of lymphoid aggregates and the small intestine revealed marked loss of villi with replacement by fibrin, necrotic debris, high numbers of macrophages and lymphocytes .All these alterations were suggested to interfere with the immunity and normal digestive processes resulting in poor weight gain and stunting of chicks. 

DOI

10.21608/ejceh.2016.247635

Keywords

Reovirus, PCR, Sequence, chicken, hematobiochemical alteration, Pathology

Authors

First Name

Neven

Last Name

Ramzy

MiddleName

M.

Affiliation

Institute of Animal health research, Dokki, Egypt. Department of Virology

Email

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City

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Orcid

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First Name

Hala

Last Name

Ibrahim

MiddleName

N.

Affiliation

Institute of Animal health research, Dokki, Egypt Department of Clinical pathology, Ismailia branch

Email

-

City

-

Orcid

-

First Name

Seham

Last Name

ElHadad

MiddleName

F.

Affiliation

Institute of Animal health research, Dokki, Egypt, Department of Pathology, Tanta Branch

Email

-

City

-

Orcid

-

Volume

2

Article Issue

2

Related Issue

33292

Issue Date

2016-11-01

Receive Date

2022-07-03

Publish Date

2016-11-01

Page Start

167

Page End

182

Online ISSN

2357-1039

Article File

EJCEH_Volume 2_Issue 2_Pages 167-182.pdf

PDF . 1.7MB

Link

https://ejceh.journals.ekb.eg/article_247635.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=247635

Order

247,635

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Molecular Characterization and Hemato Biochemical Studies of Reovirus in Ismailia Farms

Details

Type

Article

Created At

23 Jan 2023