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232402

Effect of Cinnamaldhyde on survival of E. coli O157:H7 in minced meat by using PMA- real time PCR

Article

Last updated: 29 Dec 2024

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Subjects

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Tags

Chemical Contaminants of food of animal origin
Effect of drugs and insecticides on animal health

Abstract

The antimicrobial effect of cinnamaldehyde on Escherichia coli O157:H7 in ground beef was investigated by inoculation of Escherichia coli O157:H7 into ground beef at 6 logs CFU/g, followed by addition of cinnamaldehyde (0% v/v, 0.3% v/v and 0.6% v/v). The inoculated ground beef was stored at 4˚C for 14 days, Escherichia coli O157:H7 count was determined by both culturing on Sorbitol Mackonkey agar (SMAC) and propidium Monoazide (PMA) real time PCR on days 0, 3, 5, 7 and 14. Cinnamaldehyde reduced the pathogen count by 3 log CFU/g in 14 days at 0.6% v/v and by 2.5 log CFU/g in 14 days at 0.3%. There were no significant differences between measuring of Escherichia coli O157:H7 count by both SMAC and PMA real time PCR. Cytotoxicity of Cinnamaldehyde were evaluated on BHK-21 cell line. Cinnamaldehyde can be best employed in the fight against Escherichia coli O157:H7 in meat products without causing any hazards to the consumer. 

DOI

10.21608/ejceh.2016.232402

Authors

First Name

Asmaa

Last Name

Fayed

MiddleName

M. Sh.

Affiliation

Food hygiene department, Animal Health Research Institute, Egypt

Email

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City

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Orcid

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Volume

2

Article Issue

1

Related Issue

33291

Issue Date

2016-05-01

Receive Date

2022-04-21

Publish Date

2016-05-01

Page Start

15

Page End

24

Online ISSN

2357-1039

Article File

EJCEH_Volume 2_Issue 1_Pages 15-24.pdf

PDF . 444.5kB

Link

https://ejceh.journals.ekb.eg/article_232402.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=232402

Order

232,402

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Effect of Cinnamaldhyde on survival of E. coli O157:H7 in minced meat by using PMA- real time PCR

Details

Type

Article

Created At

23 Jan 2023