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253978

Detection of aflatoxins in meat by modified HPLC method

Article

Last updated: 05 Jan 2025

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-

Tags

Chemical Contaminants of food of animal origin

Abstract

An analytical method for the determination of aflatoxins in meat was developed. Samples prepared by an extraction with dichloromethane followed by a solid phase cleanup and then fluorescence detection. Separation of the four aflatoxins(B1,G1,B2 and G2) with higher selectivity and sensitivity, and within reasonable limits of retention time, was performed. The isocratic mobile phase of water/methanol/acetonitrile (60/20/20) was used at a column temperature of 30C,with flow rate 1ml/minute and injection volume 20µl. Optimization of samples preparation and analytical conditions gave recoveries in the range of 80–90 % at spike levels of 5-40 ug/kg, and relative standard deviations (RSDs) lower than 1% in all the cases. Additionally, the limit of detection (LOD) (5ug/kg) and the limit of quantification (LOQ) (10 ug/kg) of these aflatoxins are within the legal limits set by the European Union (EU,2006).

DOI

10.21608/ejceh.2015.253978

Keywords

Aflatoxins, validation, HPLC

Authors

First Name

Mai

Last Name

Abdel Monem

MiddleName

-

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

First Name

Marwa

Last Name

Ragab

MiddleName

-

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Maher

MiddleName

Ahmed

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

First Name

Shaimaa

Last Name

Ali

MiddleName

H.

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

First Name

Nahla

Last Name

Salah

MiddleName

-

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

Hassan

MiddleName

M.

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

First Name

Fatma

Last Name

Amro

MiddleName

H.

Affiliation

Reference laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institutes, Dokki

Email

-

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

33121

Issue Date

2015-11-01

Receive Date

2022-08-10

Publish Date

2015-11-01

Page Start

945

Page End

954

Online ISSN

2357-1039

Article File

EJCEH_Volume 1_Issue 1_Pages 945-954.pdf

PDF . 240.8kB

Link

https://ejceh.journals.ekb.eg/article_253978.html

Detail API

https://ejceh.journals.ekb.eg/service?article_code=253978

Order

253,978

Type

Scientific and Research

Type Code

2,340

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry and Environmental Health

Publication Link

https://ejceh.journals.ekb.eg/

MainTitle

Detection of aflatoxins in meat by modified HPLC method

Details

Type

Article

Created At

23 Jan 2023