A study was carried out to examine the hepatonephroprotective, chromosomal protective and antioxidant potential of vitamin E + selenium alone or with charcoal against aflatoxin B1 (AFB1) toxicity in rabbits. Fifty, New Zealand white rabbits were randomly allocated into five equal groups, each of ten. Group 1 was designed as the healthy control group. Rabbits in group 2, 3, 4 and 5 were received AFB1 orally at a dose of 0.05 mg /kg of body weight daily for 10 days. Group 2 was received AFB1 alone. Group 3 was treated with vitamin E + selenium at a dose of 0.05 ml / kg b.wt. intramuscularly twice with an interval of 7 days. Group 4 was treated with activated charcoal at a dose of 0.5gm/kg feed. While, group 5 was treated with both vitamin E + selenium and activated charcoal at the same doses. Blood and tissues samples were collected from rabbits of each group on the 1st and 21th day post-treatment. The results revealed that, rabbits received AFB1 alone showed significant decrease in the RBCs, Hb, TLC, lymphocytes, neutrophils and level of antioxidant (CAT and SOD) enzymes in liver tissues with significant increase in the liver function activities(ALT, AST, ALP and LDH), urea, oxidant marker (MDA) and total chromosomal aberrations compared with the other groups. Histopathological examination showed that the main affected organ due to AFB1 toxicity was liver. Hepatic degeneration with necrosis, hyperplasia of bile duct and infiltration of leucocytic inflammatory cells were showed severely in rabbits received AFB1 alone. These parameters approximated to control levels in case of rabbits received AFB1 and treated with vitamin E +selenium either alone or with activated charcoal. The study demonstrated that administration of vitamin E + selenium alone or with activated charcoal might be useful for the treatment of aflatoxicosis in rabbits. However, activated charcoal alone was of low value