The present study was designed to evaluate the effect of ethanolic extract of Egyptian propolis in rabbits vaccinated with Pasteurella multocida, . A total of twenty clinically healthy male white New Zealand 8 weeks old rabbits were randomly divided into 4 groups each of 5 rabbits. Group (1) was injected S/C with 2mL sterile phosphate buffer saline solution (PBS) and was kept as normal control, group (2) was injected S/C with a single dose of propolis (at dose of 50 mg/kg b.wt.) , group (3) was vaccinated with Pasteurella multocida vaccine only( 1ml/kg b.wt.), and group (4) was injected S/C with both Pasteurella multocida vaccine and propolis. Treatments of propolis and Pasteurella multocida vaccine were repeated as a booster dose after three weeks. Blood samples were collected at 2 and 4 weeks post vaccination for evaluating the leukogram, immune response and serum biochemistry in all groups of animals. The result showed that propolis could enhance the antibody titer and improve cellular immune response. No statistically significant differences in serum total protein, albumin, AST, ALT activities, urea and creatinine levels were found between the control and treated groups. In conclusion, ethanolic extract of propolis administrated in combination with inactivated Pasteurella multocida vaccine was effective in improving the immune response with no adverse effects on the general health conditions in rabbits.