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244580

INDUCTION OF DEFENSE RESPONSES IN FABA BEAN PLANTS AGAINST CHOCOLATE SPOT DISEASE CAUSED BY BOTRYTIS FABAE USING CHITOSAN

Article

Last updated: 29 Dec 2024

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Subjects

-

Tags

Plant Protection and Phytopathology

Abstract

Chocolate spot disease caused by Botrytis fabae is a major problem that limits faba bean production in Egypt. The in vitro antifungal activity of chitosan and its ability to elicit defense responses in two faba bean cultivars Giza 416 (resistant) and Giza 40 (susceptible) against this fungus were evaluated. Seven isolates of B. fabae were cleaned from infected leaves. All isolates were pathogenic to Giza 40 cultivar, with isolate 3 (Bf-3) was the most virulent. Chitosan inhibits the mycelial growth of B. fabae (Bf-3) in concentration-dependent manner. At 0.75 % and 1 %, chitosan completely inhibits the growth of B. fabae. Under greenhouse conditions, two fab bean cultivars, Giza 416 (resistant) and Giza 40 (susceptible) were sprayed with 0.5% chitosan. Treatment with chitosan 0.5% reduced the chocolate spot disease severity for both cultivars compared with untreated infected control. Plants of both cultivars treated with chitosan showed a lower rate of lipid peroxidation and higher levels of chlorophyll and total phenols compared with untreated infected control. Chitosan stimulated the activity of defense enzymes such as peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). Furthermore, chitosan treatment increased the expression levels of three pathogenesis related (PR) genes including PR1, PR2 (β-1,3-glucanase), and chitinase. The resistant cultivar (Giza 461), whether treated or untreated with chitosan displayed weaker symptoms, and enhanced values for all physiological and molecular responses than the susceptible cultivar (Giza 40). The findings show that chitosan has a high potential for protecting faba bean plants from B. fabae by regulating biochemical, physiological and molecular responses in faba bean plants under infection

DOI

10.21608/jaesj.2021.244580

Keywords

chocolate spot, Botrytis fabae, Chitosan, resistance, Defense response

Authors

First Name

Shokry

Last Name

Bayoumi

MiddleName

R

Affiliation

Division of Genetics, Plant Pathology Department, Faculty of Agriculture, Damanhour University, El-Beheira, Egypt

Email

shokry_bayoumi@agr.dmu.edu.eg

City

-

Orcid

-

First Name

Asia

Last Name

Eid

MiddleName

R.

Affiliation

Plant Pathology Department, Faculty of Agriculture, Damanhour University, El-Beheira, Egypt

Email

-

City

-

Orcid

-

First Name

Ghoname

Last Name

Ammer

MiddleName

M

Affiliation

Plant Pathology Department, Faculty of Agriculture, Damanhour University, El-Beheira, Egypt

Email

-

City

-

Orcid

-

Volume

20

Article Issue

3

Related Issue

34075

Issue Date

2021-12-01

Receive Date

2022-06-18

Publish Date

2021-12-01

Page Start

32

Page End

63

Print ISSN

1687-1464

Online ISSN

2735-5098

Article File

JAESJ_Volume 20_Issue 3_Pages 32-63.pdf

PDF . 560.3kB

Link

https://jaesj.journals.ekb.eg/article_244580.html

Detail API

https://jaesj.journals.ekb.eg/service?article_code=244580

Order

244,580

Type

Full research articles

Type Code

2,324

Publication Type

Journal

Publication Title

Journal of Agricultural and Environmental Sciences

Publication Link

https://jaesj.journals.ekb.eg/

MainTitle

INDUCTION OF DEFENSE RESPONSES IN FABA BEAN PLANTS AGAINST CHOCOLATE SPOT DISEASE CAUSED BY BOTRYTIS FABAE USING CHITOSAN

Details

Type

Article

Created At

23 Jan 2023