260288

Biodegradation of plastic materials obtained from solid waste dumpsites in Nigeria, using native bacterial strains

Article

Last updated: 05 Jan 2025

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Abstract

Plastic packaging materials constitute a major potential environmental pollutant due to their slow degradation rates. This study aimed to isolate the plastic-degrading bacteria from the solid waste dumpsites of Abuja, Nigeria. Soil samples (n= 72) and plastic materials (bottles and bags) were collected from the dumpsites using soil augers and manual picking, respectively. Bacteriological analysis of the soil samples revealed the recovery of a total of 54 bacterial isolates, which were distributed among the genera of; Proteus sp. (33.3 %), Providencia sp. (29.63 %), Pseudomonas sp. (16.67 %), Bacillus sp. (9.26 %), Micrococcus sp. (5.56 %), Escherichia coli (1.85 %), Enterobacter sp. (1.85 %), and Serratia sp. (1.85 %). The bacterial isolates were inoculated into a series of shake flasks containing nutrient broth and pre-sterilized strips (1×1 cm) of plastic bags (0.05-0.0514 g) and plastic bottles (0.05-0.0529 g), and then incubated at 30 oC for 60 d to monitor their biodegradation using the weight loss method. The strips of bottles (0.58-49.00 %) were more susceptible to biodegradation than the plastic bags (0.78-15.40 %) after 60 d of incubation. The results demonstrated that about 6 of the bacterial isolates belong to the two genera of Proteus sp. and Providencia spp., and were considered the best bio-degraders. Molecular characterization of these potent isolates has identified them as Proteus mirabilis strain PPB3 (49.00 %), Proteus mirabilis strain UPMSD3 (32.07 %), Proteus mirabilis strain HH133 (20.41 %), Proteus mirabilis strain SSBIKEN (15.40 %), Providencia vermicola strain M4 (14.96 %), and Providencia vermicola strain 11 (12.20 %). These strains could be considered as potential biodegradation agents for the plastic materials that are prevalent in dumpsites.

DOI

10.21608/nrmj.2022.260288

Keywords

biodegradation, Dumpsites plastic materials, Proteus sp, Providencia sp

Authors

First Name

Beatrice

Last Name

Ojiego

MiddleName

O

Affiliation

Department of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, Nigeria

Email

beatriceojiego@yahoo.com

City

Abuja

Orcid

0000-0001-6703-4940

First Name

Obianuju

Last Name

P. Ilo

MiddleName

-

Affiliation

Department of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, Nigeria

Email

-

City

-

Orcid

0000-0003-3951-3826

First Name

Fatima

Last Name

Dantanko

MiddleName

-

Affiliation

Department of Environmental Biotechnology and Bio-conservation, National Biotechnology Development Agency, Abuja, Nigeria

Email

fatimadantanko@gmail.com

City

-

Orcid

0000-0001-5755-6016

First Name

Shauibu

Last Name

A. Abdullahi

MiddleName

-

Affiliation

Department of Biology, Ahmadu Bello University, Zaria, Kaduna, Nigeria

Email

-

City

-

Orcid

-

First Name

Ibrahim

Last Name

M. K. Gadzama

MiddleName

-

Affiliation

Department of Biology, Ahmadu Bello University, Zaria, Kaduna, Nigeria

Email

-

City

-

Orcid

-

First Name

Paul

Last Name

Bolorunduro

MiddleName

-

Affiliation

Department of Biology, Ahmadu Bello University, Zaria, Kaduna, Nigeria

Email

paulbolorunduro@gmai.com

City

-

Orcid

-

First Name

Elijah

Last Name

Ella

MiddleName

-

Affiliation

Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna, Nigeria

Email

elijahella@gmail.com

City

-

Orcid

-

First Name

Gideon

Last Name

I Ogu

MiddleName

-

Affiliation

Department of Microbiology, Federal University Lokoja, Kogi State, Nigeria

Email

-

City

-

Orcid

0000-0002-4594-8386

Volume

6

Article Issue

5

Related Issue

36634

Issue Date

2022-09-01

Receive Date

2022-08-19

Publish Date

2022-09-01

Page Start

1,713

Page End

1,724

Print ISSN

2537-0286

Online ISSN

2537-0294

Link

https://nrmj.journals.ekb.eg/article_260288.html

Detail API

https://nrmj.journals.ekb.eg/service?article_code=260288

Order

260,288

Type

Original Article

Type Code

2,265

Publication Type

Journal

Publication Title

Novel Research in Microbiology Journal

Publication Link

https://nrmj.journals.ekb.eg/

MainTitle

Biodegradation of plastic materials obtained from solid waste dumpsites in Nigeria, using native bacterial strains

Details

Type

Article

Created At

23 Jan 2023