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227879

CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt

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Last updated: 28 Dec 2024

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Abstract

Colistin resistance (Col-R) has been rising worldwide with high rate of morbidity and mortality. The emergence of mobile colistin resistance (mcr) harboring microorganisms become of a health concern. Hence, screening for fecal carriage of Col-R Enterobacteriaceae could aid in the prevention and control efforts of Col-R. This study aimed to screen for Col-R Enterobacteriaceae in the stool specimens of the hospitalized patients; explore for colistin minimal inhibitory concentrations (MIC) and the genetic determinants of these isolates, and to predict the risk factors among the studied patients' groups. Stool specimens from 290 hospitalized adult patients were screened for the presence of Col-R bacterial isolates using CHROMagar COL-APSE medium. Colistin MIC was estimated for Col-R Enterobacteriaceae using the broth microdilution (BMD) assay. Bacterial isolates were screened through the Polymerase chain reaction (PCR) for the existence of mcr-1 and mcr-2 genes. The fecal carriage of Col-R among the studied patients was 16.8 %. About 72 Col-R bacterial isolates were recovered. Col-R Enterobacteriaceae were predominant and were detected in 89.7 % of the bacterial isolates. Using the BMD, Col-R was confirmed and most of the isolates showed low resistance MIC titer (4 μg/ ml; 55.7 %). In addition, mcr-1 gene was the most frequent Col-R gene detected (69.2 %), while mcr-2 gene was less prevalent (11.5 %). The current study reported high prevalence of the Col-R and mcr-1 gene harbored by the fecal flora; with the risk to be easily transmitted inside the hospitals and within the different communities. This highlights the need for active surveillance in addition to the infection control programs.

DOI

10.21608/nrmj.2021.204801

Keywords

Colistin resistance, Fecal Carriage, Mobile colistin resistance genes, Enterobacteriaceae, mcr-1 gene

Authors

First Name

Noha

Last Name

M. Mahmoud

MiddleName

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Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

nohamostafa_81@yahoo.com

City

-

Orcid

0000-0002-6982-1216

First Name

Samah

Last Name

S. El-Kazzaz

MiddleName

-

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Email

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City

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Orcid

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Volume

5

Article Issue

6

Related Issue

32652

Issue Date

2021-12-01

Receive Date

2021-10-03

Publish Date

2021-12-01

Page Start

1,415

Page End

1,430

Print ISSN

2537-0286

Online ISSN

2537-0294

Link

https://nrmj.journals.ekb.eg/article_227879.html

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https://nrmj.journals.ekb.eg/service?article_code=227879

Order

227,879

Type

Original Article

Type Code

2,265

Publication Type

Journal

Publication Title

Novel Research in Microbiology Journal

Publication Link

https://nrmj.journals.ekb.eg/

MainTitle

CHROMagar COL-APSE medium as a screening method for fecal carriage of Colistin resistant Enterobacteriaceae among patients in Mansoura university hospitals, Egypt

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Article

Created At

23 Jan 2023