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Application of bacteriophages isolated from sewage to control urinary O157:H7 Escherichia coli and several bacterial uropathogens

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Last updated: 05 Jan 2025

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Abstract

Urinary tract infections (UTI) are global bacterial infections. Since the spread of antibiotic resistance, it is necessary to find alternative antimicrobial agents. The aim of this work was to investigate the effect of waste water bacteriophages on the growth of some multi-drug resistance Escherichia coli and other bacterial uropathogens. Urine samples were collected from 30 UTI patients at Minia University Hospital, Minia, Egypt. Twenty Escherichia coli strains were isolated from UTI patients. The Kirby-Bauer disc diffusion method was used to determine the isolates antibiotic susceptibility. The isolates showed resistance to ciprofloxacin and levofloxacin by 70%. Five E. coli bacteriophages were isolated from sewage water samples, tested for their host range and then examined by Transmission electron microscopy (TEM). The TEM examination revealed T4 like bacteriophages. The bacteriophages demonstrated lytic activities against the tested multidrug resistant clinical uropathogenic O157:H7 and non-O157 E. coli isolates, E. coli O157:H7 ATCC 43894, E. coli NRRL B-3008 and, Pseudomonas aeruginosa ATCC 27853 strain, but showed no activity against Klebsiella pneumoniae ATCC10031 and Staphylococcus aureus ATCC 6538. This study revealed that bacteriophages could act as effective alternatives of antibiotics especially against multidrug resistant bacteria; however, further in-vivo and shelf stability studies are needed.

DOI

10.21608/nrmj.2020.118451

Keywords

Urinary tract infection, Bacteriophages, Multidrug resistant bacteria, Escherichia coli O157:H7

Authors

First Name

Salwa

Last Name

M. Masoud

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt

Email

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City

-

Orcid

-

First Name

Hend

Last Name

A. Refat

MiddleName

-

Affiliation

Faculty of Science, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Nada

Last Name

S. Sayed

MiddleName

-

Affiliation

Faculty of Science, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

K. Abd-El Aal

MiddleName

-

Affiliation

Faculty of Science, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

A. Dosocky

MiddleName

-

Affiliation

Faculty of Science, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Zeinab

Last Name

S. Mohammed

MiddleName

-

Affiliation

Faculty of Pharmacy, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Mohamed

Last Name

A. H. Abdel Wahab

MiddleName

-

Affiliation

Faculty of Pharmacy, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Dina

Last Name

M. Mekawy

MiddleName

-

Affiliation

Faculty of Pharmacy, Minia University, 61519, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Ali

Last Name

I. Ali

MiddleName

-

Affiliation

Faculty of Pharmacy, Deraya University, 11566, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Basma

Last Name

A. Atya

MiddleName

-

Affiliation

Faculty of Pharmacy, Deraya University, 11566, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Katren

Last Name

T. Welliam

MiddleName

-

Affiliation

Faculty of Pharmacy, Deraya University, 11566, Minia, Egypt

Email

-

City

-

Orcid

-

First Name

Rehab

Last Name

M. Abd El-Baky

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, 11566, Minia, Egypt

Email

-

City

-

Orcid

0000-0001-6094-5653

First Name

Zeinab

Last Name

S. Hashem

MiddleName

-

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Minia University, 61519 Minia, Egypt

Email

zeinab.shawky@mu.edu.eg

City

-

Orcid

-

Volume

4

Article Issue

5

Related Issue

32643

Issue Date

2020-10-01

Receive Date

2020-09-20

Publish Date

2020-10-01

Page Start

1,005

Page End

1,014

Print ISSN

2537-0286

Online ISSN

2537-0294

Article File

NRMJ_Volume 4_Issue 5_Pages 1005-1014.pdf

PDF . 392.5kB

Link

https://nrmj.journals.ekb.eg/article_227810.html

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https://nrmj.journals.ekb.eg/service?article_code=227810

Order

227,810

Type

Original Article

Type Code

2,265

Publication Type

Journal

Publication Title

Novel Research in Microbiology Journal

Publication Link

https://nrmj.journals.ekb.eg/

MainTitle

Application of bacteriophages isolated from sewage to control urinary O157:H7 Escherichia coli and several bacterial uropathogens

Details

Type

Article

Created At

23 Jan 2023