Hospital environment and inanimate surfaces are considered as potential sources of opportunistic and nosocomial pathogens. Indirect transmission of microbes from the hospital surfaces has a major role in hospital acquired nosocomial infections and their colonization. The present study was designed to investigate the prevalence of major pathogenic bacteria isolated from hospital inanimate surfaces. Random swab samples were taken from inanimate surfaces and apparatus used in daily treatment of patients from the major hospitals in district of Faisalabad, Pakistan. These swab samples were cultivated on suitable culture media including; nutrient agar, MacConkey's agar and blood agar to isolate the bacterial pathogens. Identification of the bacterial cultures was carried out by observing the cultural and macroscopic characteristics including Gram staining. Further verification of these bacterial cultures was carried out using appropriate biochemical assays. The biochemical assays were performed for characterization of Staphylococcus aureus, Staph. epidermidis, Staph. saprophyticus, Pseudomonas aeruginosa, Klebsiella pneumonia and Escherichia coli.  These pure cultures were then tested for resistance to different antibiotics using the Kirby–Bauer method. The prevalence of Gram positive bacteria (60.83%) was more than that of Gram negative bacteria (24.13%). The results of antibiotics susceptibility tests were analyzed statistically.