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227474

Race and biovar determination of Ralstonia solanacearum in the north west of Pakistan

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Last updated: 28 Dec 2024

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Abstract

Multiple comprehensive surveys were conducted during 2012, in order to know the current status of bacterial wilt (BW) of tomato caused by Ralstonia solanacearum (R. solanacearum), in the commercial tomato growing districts of Khyber Pakhtunkhwa (KP), Pakistan. A total of 74 locations covering all the 26 districts of the 7 divisions of KP were visited for the presence of tomato plants showing BW symptoms. According to Polymerase chain reaction (PCR), the expected 281bp band was amplified from 25 candidates of R. solanacearum isolates, thus genetically confirming them to be R. solanacearum. These R. solanacearum isolates were subjected to race identification and biovar determination tests. Race differentiation was done using hypersensitive response (HR) test on tobacco plants; whereas 10% solutions of six different sugars including disaccharides (i.e. sucrose, lactose and maltose), and sugar alcohols (i.e. manitol, sorbitol and dulcitol) in Triphenyl tetrazolium chloride (TTC) medium, were used for biovars determination. Results indicated that all PCR-confirmed R. solanacearum isolates belonged to race-1 and biovar-3. However, two isolates i.e. (R. solanacearum11-DIK1 and R. solanacearum10-MDN2) were found to belong to race-3, biovar-2.

DOI

10.21608/NRMJ.2018.22706

Keywords

Tomato, Ralstonia solanacearum, PCR, Race, biovar determination

Authors

First Name

Muhammad

Last Name

Junaid

MiddleName

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Affiliation

Department of Plant Pathology, University of Agriculture, Peshawar, Pakistan

Email

mjunaid27@yahoo.com

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First Name

Musharaf

Last Name

Ahmad

MiddleName

-

Affiliation

Department of Plant Pathology, University of Agriculture, Peshawar, Pakistan

Email

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City

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Orcid

-

First Name

Saifullah

Last Name

A.

MiddleName

-

Affiliation

Department of Plant Pathology, University of Agriculture, Peshawar, Pakistan

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Volume

2

Article Issue

6

Related Issue

32605

Issue Date

2018-12-01

Receive Date

2018-11-27

Publish Date

2018-12-01

Page Start

138

Page End

146

Print ISSN

2537-0286

Online ISSN

2537-0294

Link

https://nrmj.journals.ekb.eg/article_227474.html

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https://nrmj.journals.ekb.eg/service?article_code=227474

Order

227,474

Type

Original Article

Type Code

2,265

Publication Type

Journal

Publication Title

Novel Research in Microbiology Journal

Publication Link

https://nrmj.journals.ekb.eg/

MainTitle

Race and biovar determination of Ralstonia solanacearum in the north west of Pakistan

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Article

Created At

23 Jan 2023